Proline transport by brush-border membrane vesicles of lobster antennal glands

Journal Article · Thu Feb 01 04:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:6853937

Behnke, R D; Wong, R K; Huse, S M; Reshkin, S J; Ahearn, G A ^[1]

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1. Univ. of Hawaii, Manoa, Honolulu (USA)

Purified brush-border membrane vesicles (BBMV) of lobster antennal gland labyrinth and bladder were separately formed by a magnesium precipitation technique. L-(3H)proline uptake was stimulated by a transmembrane NaCl gradient (outside (o) greater than inside (i)) to a greater extent in BBMV from labyrinth than those from the bladder. Detailed study of the labyrinth proline-transport processes revealed a specific dependence on NaCl, with negligible stimulatory effects by NaSCN, Na-gluconate, or KCl. A transmembrane proton gradient (o greater than i) was without stimulatory effect on proline transport. A transmembrane potential difference alone, in the presence of equilibrated NaCl and L-(3H)proline, led to net influx of the labeled amino acid, suggesting that the uptake process was electrogenic and capable of bringing about the net transfer of positive charge to the vesicle interior. Although a transmembrane Na gradient alone, in the presence of equilibrated Cl and L-(3H)proline, was able to bring about the net influx of the amino acid, a transmembrane Cl gradient alone under Na- and L-(3H)proline-equilibrated conditions was not, suggesting that only the Na gradient could energize the carrier process through cotransport, while the anion served an essential activating role. Proline influx by these vesicles occurred by the combination of at least one saturable Michaelis-Menten carrier system (apparent Kt = 0.37 mM; apparent JM = 1.19 nmol.mg protein-1.10 s-1) and apparent diffusion (P = 0.33 nmol.mg protein-1.10 s-1.mM-1). Static head analysis of the transport process suggested a cotransport stoichiometry of 2 Na:1 proline with essential activation by Cl ion.

• OSTI ID: 6853937
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 258; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
ANIMALS
ANIONS
AQUATIC ORGANISMS
ARTHROPODS
AZOLES
BARYONS
BLADDER
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
CRUSTACEANS
DECAPODS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INVERTEBRATES
IONS
ISOTOPE APPLICATIONS
LOBSTERS
MEMBRANE TRANSPORT
MEMBRANES
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROLINE
PROTONS
PYRROLES
PYRROLIDINES
SODIUM CHLORIDES
SODIUM COMPOUNDS
STOICHIOMETRY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINARY TRACT