Inhibition of Na -P/sub i/ cotransporter in small gut brush border by phosphonocarboxylic acids

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:6411864

Loghman-Adham, M; Szczepsanska-Konkel, M; Yusufi, A N.K.; Van Scoy, M; Dousa, T P

The authors examined the effect of phosphonoformic acid (PFA) and phosphonoacetic acid (PAA) upon Na -P/sub i/ cotransport in brush-border membrane (BBM) from small gut of rat. Both PFA and PAA inhibited the Na gradient-dependent uptake of TSP/sub i/ by BBM vesicles (BBMV) prepared from intestinal mucosa but had no effect on Na -dependent uptakes of D-(TH)glucose, L-(TH)proline, or ( UC)succinate. The uptake in the absence of Na gradient, or uptake at equilibrium period (180 min), was not affected by PFA or by PAA. A chemical analogue of PFA and PAA, phosphonopropionic acid, had only a minor inhibitory effect and phenylphosphonic acid was inactive. Neither PFA nor PAA influenced the activity of rat intestinal BBM alkaline phosphatase. The BBMV from rat jejunum had a much higher capacity for Na gradient-dependent uptake of TSP/sub i/ than BBMV from duodenum or ileum. The inhibition of BBMV TSP/sub i/ transport across rat jejunum by PFA is competitive. They suggest that PFA and PAA are specific inhibitors of Na gradient-dependent uptake of P/sub i/ by BBMV from small intestinal mucosa and that they could serve as useful experimental tools for the studies of intestinal Na -P/sub i/ cotransport.

• Research Organization: Mayo Clinic and Foundation, Rochester, MN
• OSTI ID: 6411864
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:2; ISSN AJPHA
• Country of Publication: United States
• Language: English

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ALDEHYDES
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
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CHARGED PARTICLES
CHEMISTRY
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DICARBOXYLIC ACIDS
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GLUCOSE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
INTESTINES
IONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MEMBRANE TRANSPORT
MEMBRANES
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
MUCOUS MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHONIC ACIDS
PHOSPHORUS 32
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PROLINE
PYRROLES
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