Transepithelial movement of non-polar and polar compounds in male rat reproductive tubule examined by in vivo microperifusion and in vivo micropuncture

Journal Article · Sat Mar 31 23:00:00 EST 1990 · Journal of Urology; (USA)

OSTI ID:6853918

Yamamoto, M; Turner, T T ^[1]

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1. Univ. of Virginia School of Medicine, Charlottesville (USA)

Proluminal movement of {sup 3}H-testosterone and {sup 3}H-sucrose from peritubular to intratubular fluids of the adult rat testis and epididymis was investigated by using in vivo microperifusion and subsequent micropuncture of seminiferous tubules and caput, corpus, and cauda epididymal tubules. Tubules were perifused with Minimum Essential Medium containing {sup 3}H-testosterone or {sup 3}H-sucrose. {sup 14}C-polyethyleneglycol was included in the perifusion fluid as a marker for contamination of intraluminal fluid by interstitial fluid. Radioactivity of isotopes in perifusion and intraluminal fluids was determined at one and two hours after perifusion and the percentage of peritubular isotopes appearing in intraluminal fluid was determined. Net entry of {sup 3}H-sucrose into the seminiferous and epididymal tubules was significantly reduced. Proluminal movement of {sup 3}H-androgen across the seminiferous epithelium was also restricted. In contrast, intraluminal {sup 3}H-androgen concentrations in caput epididymal fluid were 200 to 300% of those in peritubular fluid at both one and two hours after perifusion. Similar results were found in the corpus epididymidis. {sup 3}H-androgen concentrations in cauda epididymal fluid were approximately 125% of peritubular isotope concentrations. The exact mechanism underlying this uphill proluminal movement of 3H-androgen into the rat epididymal lumen remains to be elucidated.

• OSTI ID: 6853918
• Journal Information: Journal of Urology; (USA), Journal Name: Journal of Urology; (USA) Vol. 143:4; ISSN JOURA; ISSN 0022-5347
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
DISACCHARIDES
EPITHELIUM
GONADS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IN VIVO
ISOTOPE APPLICATIONS
KETONES
MALE GENITALS
MAMMALS
MEMBRANE TRANSPORT
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PERFUSED TISSUES
RATS
RODENTS
SACCHARIDES
SACCHAROSE
STEROID HORMONES
STEROIDS
TESTES
TESTOSTERONE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES