Serum copper concentration as an index of experimental lung injury

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Advances in Experimental Medicine and Biology; (USA)

DOI:https://doi.org/10.1007/978-1-4613-0537-8_26· OSTI ID:6853399

Ward, W F; Molteni, A; Ts'ao, C; Ischiropoulos, H ^[1]

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1. Northwestern Univ. Medical School, Chicago, IL (USA)

Serum copper (Cu) concentration was evaluated as an index of lung injury in two rat models of pneumotoxicity: hemithoracic irradiation and monocrotaline ingestion. In both models there was a dose- and time-dependent increase in serum Cu concentration. This hypercupremia paralleled the development of pulmonary endothelial dysfunction (decreased lung plasminogen activator activity and increased prostacyclin production) and pulmonary fibrosis (hydroxyproline accumulation). In the radiation model, lung injury and hypercupremia persisted for at least 6 months, and were spared similarly when the total dose was delivered in multiple daily fractions as compared to single doses. In irradiated rats, the elevated serum Cu concentration was accompanied by increases in plasma ceruloplasmin, lung Cu concentration, and lung Cu/Zn superoxide dismutase (SOD) activity. In monocrotaline-treated rats, lung damage and hypercupremia also were accompanied by a reduction in liver Cu concentration, and by a direct correlation between the concentrations of Cu and SGOT in the serum. In both models, some but not all modifiers of lung damage (penicillamine, angiotensin converting enzyme inhibitors, pentoxifylline) also partially prevented the insult-induced hypercupremia. In contrast, serum iron concentration was largely independent of treatment in all experiments. These data suggest that elevated serum copper concentration is an accurate and minimally invasive index of lung injury in irradiated and monocrotaline-treated rats.

• OSTI ID: 6853399
• Journal Information: Advances in Experimental Medicine and Biology; (USA), Journal Name: Advances in Experimental Medicine and Biology; (USA) Vol. 258; ISSN 0065-2598; ISSN AEMBA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
BIOLOGICAL RADIATION EFFECTS
BODY
COPPER
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FIBROSIS
INJURIES
IRRADIATION
LUNGS
MAMMALS
METABOLISM
METALS
ORGANS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
PROSTAGLANDINS
RADIATION EFFECTS
RADIATION INJURIES
RATS
RESPIRATORY SYSTEM
RODENTS
SUPEROXIDE DISMUTASE
TIME DEPENDENCE
TRANSITION ELEMENTS
VERTEBRATES