Disposition of naphthalene and its metabolites in the brain of rainbow trout (Salmo gairdneri)

Journal Article · Wed Oct 01 04:00:00 EDT 1980 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(80)90091-2· OSTI ID:6852015

Collier, T K; Krahn, M M; Malins, D C

Rainbow trout (Salmo gairdneri) were exposed to orally administered (/sup 3/H)naphthalene. Another group received naphthyl glucuronic acid and naphthyl sulfate via iv injection. Brain, liver, and blood were assayed for the parent compound and/or total metabolites. Individual naphthalene derivatives were determined by high-performance liquid chromatography (hplc) using either radiometric or on-line fluorimetric detection systems. Naphthalene concentrations in brain (8.2 pmol/mg dry wt at 16 hr after feeding) approximated those found at the same time in liver (7.4 pmol/mg dry wt). A nonconjugated naphthalene derivative, 1,2-dihydro-1,2-dihydroxynaphthalene, also accumulated in brain (0.041 pmol/mg dry wt after 16 hr), although to a lesser degree than in liver (0.10 pmol/mg dry wt after 16 hr). Conjugated naphthalene derivatives, 1-naphthyl sulfate and 1-naphthyl glucuronic acid, although present in liver and blood, were largely excluded from the brain. Low naphthalene hydroxylase activity (<2.0 pmol product formed/mg protein/min) indicated that the trout brain has a minimal ability to oxidize aromatic hydrocarbons. These findings suggest that the brain of adult trout is substantially different from other tissues (e.g., liver and blood) with respect to the disposition of naphthalene and its metabolites.

• Research Organization: National Marine Fisheries Service, Seattle, WA
• OSTI ID: 6852015
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 23:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

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560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
CONTAMINATION
DATA
DIGESTIVE SYSTEM
DISTRIBUTION
EXPERIMENTAL DATA
FISHES
GLANDS
GLUCURONIC ACID
HYDROCARBONS
HYDROXY ACIDS
INFORMATION
INJECTION
INTAKE
INTRAVENOUS INJECTION
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MATERIALS
METABOLITES
NAPHTHALENE
NERVOUS SYSTEM
NUMERICAL DATA
ORAL ADMINISTRATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
QUANTITATIVE CHEMICAL ANALYSIS
RETENTION
SEPARATION PROCESSES
TIME DEPENDENCE
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
TROUT
VERTEBRATES