Metabolism of orally administered naphthalene in spawning english sole (Parophrys vetulus)

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90087-1· OSTI ID:5530122

Reichert, W L; Varanasi, U

English sole (gravid females and ripe males) were force-fed (/sup 3/H)naphthalene (NPH) in salmon oil and tissues were examined at 24, 48, and 168 hr. NPH was detected in all tissues and fluids examined (e.g., liver, blood, muscle, bile, testes, and ovaries) at 24 hr. There were no statistically significant differences in NPH concentrations, based on dry weight, between comparable tissues and fluids of the sexes, except for the gonads. Ovarian NPH concentrations were approximately three times higher than those in testes. The highest percentage (5%) of the total dose at 24 hr was present in the ovaries; liver and muscle contained about 1-2% of the dose. Concentrations of NPH in all tissues declined markedly from 24 to 168 hr. At 24 hr, male English sole had significantly higher hepatic NPH hydroxylase activity than did female sole. However, no significant sex differences were evident in tissue concentrations of total metabolites of NPH. In liver, blood, and testes, the metabolites at 24 hr were composed of approximately equal proportions of conjugates and nonconjugates. Nonconjugates were the predominant metabolites in the ovaries (>88% of the total). Thin-layer chromatography (TLC) of metabolites from liver, blood, ovaries, and testes revealed that the major nonconjugate metabolite was 1,2-dihydro-1,2-dihydroxynaphthalene (diol) and glucuronides were the major conjugate class present. Both TLC and high-performance liquid chromatography indicated that the glucuronide conjugate in the liver was derived from the diol. The naphthol conjugates were not detected. The ability of fish to rapidly accumulate orally administered NPH and its metabolites in the gonads may have significant implications with respect to fertilization and subsequent embryo development.

• Research Organization: Northwest and Alaska Fisheries Center, Seattle, WA
• OSTI ID: 5530122
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 27:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BILE
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
ENZYMES
FEMALE GENITALS
FISHES
GLANDS
GONADS
HYDROCARBONS
HYDROXYLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MALE GENITALS
MATERIALS
METABOLISM
METABOLITES
MUSCLES
NAPHTHALENE
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANS
OVARIES
OXIDOREDUCTASES
TESTES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES