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Effects of steroids and sex reversal on intestinal absorption of L-(/sup 14/C)leucine in vivo, in rainbow trout, Salmo gairdneri

Journal Article · · Gen. Comp. Endocrinol.; (United States)
;
The effects of steroids (17 alpha-methyltestosterone (MT), 17 beta-oestradiol (E2)), and of sex reversal (XX male) on intestinal absorption and accumulation of L-(/sup 14/C)leucine (5 mM), were investigated in unanaesthetized rainbow trout (Salmo gairdneri), using an in vivo gut perfusion technique. Each steroid was luminally perfused through the gut at a concentration of 50 micrograms/ml perfusate, during five separate perfusions carried out on the same fish at 30-min intervals (perfusion periods 1 to 5), for a total of 120 min at 14 degrees. Experiments were also conducted on masculinized, genetically female trout (XX male) with steroid-free perfusate. MT treatment significantly increased the intestinal absorption of radioleucine during periods 1 and 2, whilst E2 was without effect. Neither MT nor E2 influenced intestinal accumulation (mid- and hindgut) of radioleucine, and accumulation of /sup 14/C-solutes in skeletal muscle. Sex reversal, however, whilst having no effect on leucine absorption, nevertheless significantly increased intestinal accumulation of radioleucine, and accumulation of /sup 14/C-solutes in skeletal muscle. The effects observed in the present study are in agreement with previous work in trout using everted gut sac preparations. It is suggested that the growth-promoting effects of anabolic-androgenic steroids in fish may be partly explained by their action on gastrointestinal function.
Research Organization:
Department of Biological Sciences, University of Aston, Birmingham, England
OSTI ID:
6851495
Journal Information:
Gen. Comp. Endocrinol.; (United States), Journal Name: Gen. Comp. Endocrinol.; (United States) Vol. 52:3; ISSN GCENA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550801 -- Morphology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AMINO ACIDS
ANDROGENS
ANDROSTANES
ANIMAL GROWTH
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
ESTRADIOL
ESTRANES
ESTROGENS
FEMALES
FISHES
GROWTH
HORMONES
HYDROXY COMPOUNDS
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEUCINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
STEROID HORMONES
STEROIDS
TESTOSTERONE
TRACER TECHNIQUES
TROUT
UPTAKE
VERTEBRATES