Uptake, metaolism, and elimination of /sup 14/C-labelled 1,2,4-trichlorobenzene in rainbow trout and carp

Journal Article · Thu May 01 04:00:00 EDT 1980 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398009529882· OSTI ID:6849983

Melancon, M J; Lech, J J

Fingerling rainbow trout (Salmo gairdneri) were exposed to /sup 14/C-labeled 1,2,4-trichlorobenzene (TCB) for 8 h in a static exposure (0.018 mg/l) or for 35 d in a continuous-flow exposure (0.020 mg/l) followed by a subsequent elimination period. For the 2 d after the 8-h exposure, the half-time (t/sub 1/2) of elimination of /sup 14/C from muscle and liver was 0.4 d, while after the 35-d exposure an early rapid elimination of /sup 14/C from these tissues (t/sub 1/2/ = 0.4 d) was followed by a slower elimination (t/sub 1/2/ = 50 d) during d 4 to 36. The maximum bioconcentration factors for /sup 14/C in muscle and liver were 51 and 102 after the 8-h exposure and 89 and 389 during the 35-d exposure. The values for bile were much greater, reaching 240 after the 8-h exposure and 1400 during the 35-d exposure. When larger trout and carp (Cyprinus carpio) were exposed to (/sup 14/C)TCB (0.2 to 0.4 mg/l) the bioconcentration factor for bile /sup 14/C to water /sup 14/C was less than 100. Pretreatment of trout with BETA-naphthoflavone, an inducer of hepatic mixed-function oxidase, increased this bioconcentration factor for bile to several hundred. Solvent partitioning and thin-layer chromatography (TLC) indicated that about 60% of the /sup 14/C in bile of control trout or carp was present as highly polar biotransformation products, while for induced trout the value was more than 90%. TLC in two solvent systems suggested that at least two such products were present in bile from control fish and at least three in bile from induced fish. About half of the /sup 14/C in bile from induced trout was more polar than the /sup 14/C in bile from normal trout.

• Research Organization: Univ. of Wisconsin, Milwaukee
• OSTI ID: 6849983
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 6:3; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BILE
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
CLEARANCE
DIGESTIVE SYSTEM
FISHES
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
LIVER
MATERIALS
METABOLISM
MUSCLES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
SEPARATION PROCESSES
TROUT
VERTEBRATES