Computerized optimization of radioimmunoassays for hCG and estradiol: an experimental evaluation
The mathematical and statistical theory of radioimmunoassays (RIAs) has been used to develop a series of computer programs to optimize sensitivity or precision at any desired dose level for either equilibrium or nonequilibrium assays. These computer programs provide for the calculation of the equilibrium constants of association and binding capacities for antisera (parameters of Scatchard plots), the association and dissociation rate constants, and prediction of optimum concentration of labeled ligand and antibody and optimum incubation times for the assay. This paper presents an experimental evaluation of the use of these computer programs applied to RIAs for human chorionic gonadotropin (hCG) and estradiol. The experimental results are in reasonable semiquantitative agreement with the predictions of the computer simulations (usually within a factor of two) and thus partially validate the use of computer techniques to optimize RIAs that are reasonably well behaved, as in the case of the hCG and estradiol RIAs. Further, these programs can provide insights into the nature of the RIA system, e.g., the general nature of the sensitivity and precision surfaces. This facilitates empirical optimization of conditions.
- Research Organization:
- National Inst. of Health, Bethesda, MD
- OSTI ID:
- 6849960
- Journal Information:
- Anal. Biochem.; (United States), Vol. 88:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
COMPUTER CODES
E CODES
K CODES
R CODES
S CODES
ESTRADIOL
RADIOIMMUNOASSAY
HCG
OPTIMIZATION
ANTIGEN-ANTIBODY REACTIONS
COMPARATIVE EVALUATIONS
IMMUNE SERUMS
IODINE 131
LABELLED COMPOUNDS
MAN
SIMULATION
TRITIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ESTRANES
ESTROGENS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
RADIOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics