Differential inhibition of the rejoining of x-ray-induced DNA strand breaks in normal and transformed human fibroblasts treated with 1,3-bis(2-chloroethyl)-1-nitrosourea in vitro

Journal Article · Sun Jan 01 00:00:00 EST 1978 · Cancer Res.; (United States)

OSTI ID:6848339

Erickson, L C; Bradley, M O; Kohn, K W

The effects of 1,3-bis(2-chloroethyl)-1-nitrosourea on the rejoining of x-ray-induced DNA strand breaks were examined in normal human fibroblasts (WI-38) and a simian virus 40-transformed derivative (VA-13) with the use of alkaline sucrose sedimentation. 1,3-Bis(2-chloroethyl)-1-nitrosourea was capable of partially inhibiting repair of x-ray-produced DNA strand breaks in both cell types when the drug was added to the culture medium immediately after x-irradiation. However, when 1,3-bis(2-chloroethyl)-1-nitrosourea exposure preceded x-ray by 1 hr, DNA repair was inhibited to a much greater extent than it was when 1,3-bis(2-chloroethyl)-1-nitrosourea followed x-ray. The inhibition of DNA repair by 1,3-bis(2-chloroethyl)-1-nitrosourea appeared to be complete in the transformed VA-13 cells, while only partial inhibition of repair was observed in the normal WI-38 cells.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 6848339
• Journal Information: Cancer Res.; (United States), Vol. 38:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
BIOLOGICAL REPAIR
INHIBITION
NITROSO COMPOUNDS
BIOLOGICAL EFFECTS
X RADIATION
ALKYLATION
ANIMAL CELLS
CELL CULTURES
DNA
FIBROBLASTS
IN VITRO
MAN
MONKEYS
RESPONSE MODIFYING FACTORS
STRAND BREAKS
ANIMALS
BIOLOGICAL RECOVERY
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550600 - Medicine