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Differential inhibition of the rejoining of x-ray-induced DNA strans breaks in normal and transformed human fibroblasts treated with 1,3-bis(2-chloroethyl)-1-nitrosourea in vitro

Journal Article · · Cancer Res.; (United States)
OSTI ID:5638356
; ;

The effects of 1,3-bis(2-chloroethyl)-1-nitrosourea on the rejoining of x-ray-induced DNA strand breaks were examined in normal human fibroblasts (WI-38) and a simian virus 40-transformed derivative (VA-13) with the use of alkaline sucrose sedimentation. 1,3-bis(2-chloroethyl)-1-nitrosourea was capable of partially inhibiting repair of x-ray-produced DNA strand breaks in both cell types when the drug was added to the culture medium immediately after x-irradiation. However, when 1,3-bis(2-chloroethyl)-1-nitrosourea exposure preceded x-ray by 1 h, DNA repair was inhibited to a much greater extent than it was when 1,3-bis(2-chloroethyl)-1-nitrosourea followed x-ray. The inhibition of DNA repair by 1,3-bis(2-chloroethyl)-1-nitrosourea appeared to be complete in the transformed VA-13 cells, while only partial inhibition of repair was observed in the normal WI-38 cells.

Research Organization:
National Cancer Inst., Bethesda, MD
OSTI ID:
5638356
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 38:3; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
IONIZING RADIATIONS
KINETICS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
VIRUSES
X RADIATION