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Differential inhibition of the rejoining of x-ray-induced DNA strand breaks in normal and transformed human fibroblasts treated with 1,3-bis(2-chloroethyl)-1-nitrosourea in vitro

Journal Article · · Cancer Res.; (United States)
OSTI ID:6848339
; ;

The effects of 1,3-bis(2-chloroethyl)-1-nitrosourea on the rejoining of x-ray-induced DNA strand breaks were examined in normal human fibroblasts (WI-38) and a simian virus 40-transformed derivative (VA-13) with the use of alkaline sucrose sedimentation. 1,3-Bis(2-chloroethyl)-1-nitrosourea was capable of partially inhibiting repair of x-ray-produced DNA strand breaks in both cell types when the drug was added to the culture medium immediately after x-irradiation. However, when 1,3-bis(2-chloroethyl)-1-nitrosourea exposure preceded x-ray by 1 hr, DNA repair was inhibited to a much greater extent than it was when 1,3-bis(2-chloroethyl)-1-nitrosourea followed x-ray. The inhibition of DNA repair by 1,3-bis(2-chloroethyl)-1-nitrosourea appeared to be complete in the transformed VA-13 cells, while only partial inhibition of repair was observed in the normal WI-38 cells.

Research Organization:
National Cancer Inst., Bethesda, MD
OSTI ID:
6848339
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 38:3; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

550600 -- Medicine
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
IN VITRO
INHIBITION
IONIZING RADIATIONS
MAMMALS
MAN
MONKEYS
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
STRAND BREAKS
VERTEBRATES
X RADIATION