Mechanisms of tolerance in murine radiation bone marrow chimeras. I. Nonspecific suppression of alloreactivity by spleen cells from early, but not late, chimeras

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · Transplantation; (United States)

OSTI ID:6847456

Auchincloss, H Jr; Sachs, D H

Allogeneic chimeras were prepared using lethally irradiated B6 hosts and untreated marrow from exsanguinated BALB/c donors. For about two months after reconstitution, chimeras had very weak antihost cell-mediated lymphocytotoxicity (CML) reactivity and little third-party alloreactivity. During this time a cell population capable of suppressing CML reactivity against both host and third-party alloantigens (i.e., antigen-nonspecific) was demonstrated in chimera spleens by in vitro mixing experiments. The putative suppressor cells were Thy-1-negative and radiation-sensitive. Subsequently, mature chimeras showed host tolerance and strong third-party alloreactivity. At this point suppressor mechanisms could no longer be demonstrated. These data are consistent with a clonal elimination hypothesis in that they do not provide evidence to indicate that maintenance of specific immune tolerance is mediated by an active suppressor mechanism.

• OSTI ID: 6847456
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 36:4; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BODY
BONE MARROW
CELL CULTURES
CHIMERAS
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSION
MAMMALS
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RODENTS
TISSUES
TOLERANCE
TRANSPLANTS
VERTEBRATES