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Scanning electron microscopy and recording of the physiological activity of tracheal ciliated cells treated by fractionated irradiation

Journal Article · · Scanning Electron Microsc.; (United States)
OSTI ID:6847386
; ;
The ciliated epithelium of the rabbit's trachea was irradiated with daily fractions of 2 Gy to an accumulated dose of 20 Gy. The beat frequency of the cilia was recorded and specimens were taken for SEM-, TEM- and LM-investigations. Examination was made 1-10 days after each fractionation schedule. An increased ciliary beat frequency was recorded at 2 Gy and 4 Gy. With increasing dose, there was an inverted relationship to the frequency. Light-microscopy showed edema and an increased amount of goblet cells in relation to the increasing dose. With SEM an increased number of ciliary blebs could be seen. These could be classified according to size and number, and showed a positive correlation to the dose. TEM-investigations showed signs of increased intracellular activity at higher doses in the form of multilobulated nuclei and an increasing number of nuclear pores. At lower doses, an increased amount of mitochondria appeared in the apical part of the cell. It is at present difficult to evaluate any correlation between the physiological activity and the morphology. More biological data are needed to explain the early irradiation effects.
Research Organization:
Department of Oncology, University Hospital, Lund, Sweden
OSTI ID:
6847386
Journal Information:
Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States); ISSN SEMYB
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON MICROSCOPY
EPITHELIUM
FRACTIONATED IRRADIATION
IRRADIATION
MAMMALS
MICROSCOPY
MITOCHONDRIA
ORGANOIDS
RABBITS
RADIATION EFFECTS
RESPIRATORY SYSTEM
SCANNING ELECTRON MICROSCOPY
TISSUES
TRACHEA
VERTEBRATES