Ciliated cells of the trachea of the rabbit, treated with cis-diamminedichloroplatinum (II) alone, or in combination with ionizing radiation

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Scanning Electron Microsc.; (United States)

OSTI ID:6840822

Albertsson, M; Hakansson, C H; Mercke, C

The ciliated epithelium of the rabbit trachea was irradiated with daily fractions of 2 Gy to an accumulated dose of 20 Gy (TD: 2, 6, 10, 16, or 20 Gy). Fifteen to forty-five minutes before start of the first irradiation (treatment day 1), 5 mg cis-DDP was given by intraperitoneal injection to each rabbit. Examination was made 1 - 10 days after each fractionation schedule, when specimens were taken for investigations. Scanning electron microscope investigations showed a gradual development of ciliary damage from blebs on the cilia to swollen tips, broken and bent cilia and finally an epithelial lining with areas free from cilia with a surface covered with microvilli-like structures. SEM also showed cell loss, and remnants of dead cells on the surface together with detritus. By transmission electron microscope ciliary damage, cell death and cell loss of the ciliated cell layer as well as exfoliation of portions of goblet-like cells on the surface could be confirmed. The irradiated ciliated epithelium and the untreated control epithelium in each animal showed no difference in this respect. Thus no enhancement of the effects of radiation could be observed. The development of ultrastructural damage may be due to a cytotoxic effect of the drug on the ciliated epithelium. However, 19 days after the start of cis - DDP injection, a hyperplasia of the basal cell layer was observed, which indicates that the observed cytotoxicity of the drug is reversible and a normalisation occurs during the last days of observation in this study.

• Research Organization: University Hospital, Lund, Sweden
• OSTI ID: 6840822
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States) Vol. 3; ISSN SEMYB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
DOSES
ELECTRON MICROSCOPY
ELEMENTS
EPITHELIUM
FRACTIONATED IRRADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
METALS
MICROSCOPY
MORPHOLOGICAL CHANGES
PLATINUM
PLATINUM METALS
RABBITS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RESPIRATORY SYSTEM
SCANNING ELECTRON MICROSCOPY
SYNERGISM
TISSUES
TRACHEA
TRANSITION ELEMENTS
TRANSMISSION ELECTRON MICROSCOPY
ULTRASTRUCTURAL CHANGES
VERTEBRATES