Amplification of oncogenes and integrated SV40 sequences in mammalian cells by the decay of incorporated iodine-125

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576968· OSTI ID:6846798

Ehrfeld, A; Planas-Bohne, F; Luecke-Huhle, C

Iodine-125, in the form of 5-(/sup 125/I)iododeoxyuridine (I-UdR), was incorporated into the DNA of SV40 transformed Chinese hamster embryo cells. Disintegration of the /sup 125/I led to increased cell killing with increasing dose as measured by the colony-forming ability of single cells. The D37 (the dose at which 37% of the cells survive) amounts to 95 decays per cell, corresponding to 0.66 Gy. Variations in the copy number of specific DNA sequences was measured by using dispersed cell blotting with sensitive DNA hybridizations. A 13-fold amplification of the viral DNA sequences (SV40) and a twofold amplification of two cellular oncogenes of the ras-family (Ki-ras and Ha-ras) were found. Other cellular genes, like the alpha-actin gene, were not amplified, and no variation in gene copy number was detected after incubation of cells with cold I-UdR. We suggest the observed gene amplifications are induced by the densely ionizing radiation emitted by the decay of the incorporated /sup 125/I atoms.

• Research Organization: Institut fuer Genetik und fuer Toxikologie von Spaltstoffen, Germany, F.R.
• OSTI ID: 6846798
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
CHO CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GENE AMPLIFICATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIOISOTOPES
RIBOSIDES
STRUCTURAL CHEMICAL ANALYSIS
URACILS