Nitrobenzo[[alpha]]Pyrene-induced DNA amplification in SV40-transformed Chinese hamster embryo cells

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850190210· OSTI ID:6700377

Neft, R E; Roe, A L; Schol, H M; Fu, P P; Mittelstaedt, R A; Casciano, D A ^[1]

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1. Univ. of Arkansas, Little Rock, AR (United States)

Nitrobenzo(a)pyrenes (NBaPs) are ubiquitous environmental pollutants that produce mutations in Salmonella typhimurium and Chinese hamster ovary cells. In this study, l-, 3-, and 6-NBaP induced amplification of SV40 DNA sequences in an SV40-transformed Chinese hamster embryo cell line which is sensitive to DNA amplification by various known carcinogens. Of the three isomers, 3-NBaP produced the highest level of gene amplification, which was 4.8 relative to untreated controls at a dose of 5 [mu]g/ml. Considering the relationship between gene amplification and tumorigenesis, it seems prudent to carry out a more exhaustive analysis of the carcinogenic potential of these agents.

• OSTI ID: 6700377
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 19:2; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

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