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Relation of ultraviolet light mutagenesis to a radiation-damage inducible system in Escherichia coli. [X and gamma radiation]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574615· OSTI ID:6845016
 [1]; ; ;
  1. Pennsylvania State Univ., University Park
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Revertants produced by ultraviolet light (uv) were shown to follow a square-law dependence on fluence up to about 0.5 J/m/sup 2/, after which the dependence became closer to linear. This behavior can be associated with an induction process, and a new way of expressing mutation data is presented that allows one to estimate the fraction of cells in a population that is inducible after exposure to a particular uv fluence. Comparison with two other kinds of radiation-induced behavior (induction of inhibition of post-irradiation DNA degradation and induced radioresistance) shows that the fluence dependence of induction is similar in all three cases. A predose with ionizing radiation followed by an incubation period and subsequent exposure to a graded set of uv fluences at 265 nm gave an increase in the number of revertants and a more linear dose dependence. The same result was obtained if the predose was a uv fluence at 265 nm and the graded set of doses was at 313 nm, a wavelength which by itself produces very few revertants. These changes in the mutagenic response were also consistent with the induction of a mutation-potentiating system by the predose. Ultraviolet-produced mutations in the presence or absence of an ionizing radiation predose appear to be due to C ..-->.. T changes in the bacterial genome (C and T denote cytosine and thymine, respectively).
OSTI ID:
6845016
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 72:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MICROORGANISMS
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
ULTRAVIOLET RADIATION
X RADIATION