Purification of tracer for somatomedin C radioimmunoassay by hydrophobic interaction chromatography

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6844106

Baxter, R C; Brown, A S

Researcherse have purified tracer for use in the somatomedin C radioimmunoassay by hydrophobic interaction chromatography. Material showing greatest immunoreactivity binds to Octyl Sepharose CL-4B (Pharmacia) in a buffer mixture consisting of 130 mL of acetonitrile and 870 mL of 0.1 mol/L NH4HCO3, pH 7.8, but is eluted by increasing the acetonitrile content to 180 mL/L. As compared with tracer purified by binding to specific antiserum in liquid phase, precipitating the complex with second antibody, and then dissociating by gel chromatography at acid pH, this tracer shows equal immunoreactivity against specific somatomedin C antiserum. Either preparation allows excellent discrimination between extracts of normal, acromegalic, and hypopituitary plasma samples; thus either is suitable for use in the somatomedin C radioimmunoassay. Tracer purification by hydrophobic interaction chromatography is rapid and inexpensive. It may be useful in preparing highly immunoreactive tracers for other peptide radioimmunoassays.

• Research Organization: Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, N.S.W., Australia
• OSTI ID: 6844106
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:3; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BINDING ENERGY
CHEMICAL PREPARATION
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ENERGY
IMMUNE REACTIONS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES