Immunoreactive somatomedin A in human serum

Journal Article · Thu Feb 01 04:00:00 EST 1979 · J. Clin. Endocrinol. Metab.; (United States)

DOI:https://doi.org/10.1210/jcem-48-2-271· OSTI ID:6037161

Hall, K; Brandt, J; Enberg, G; Fryklund, L

A RIA has been developed for somatomedin A (SM-A) utilizing Sepharose-bound antibodies. This assay, measuring SM-A, the insulin-like growth factors 1 and 2, and somatomedin C, allows determination in serum samples. In comparison with a serum standard, the mean serum levels in patients with acromegaly or GH deficiency and healthy subjects were 8.7 +- 0.7 (n=25), 0.24 +- 0.02 (n=25), and 1.15 +- 0.11 U/ml, respectively. The correlation coefficient between immunoreactive SM-A and SM-A by radioreceptor assay was highly significant (r=0.93), although the potency ratio of SM-A between the two groups of patients was higher in the RIA than in the radioreceptor assay. Gel chromatography revealed that SM-A in acromegalic serum is bound to a carrier protein which is absent in patients with GH deficiency. After gel chromatography at low pH, 90% of applied immunoreactive SM-A was recovered in the low molecular weight fraction and consisted mainly of neutral polypeptides.

• Research Organization: Karolinska Hospital, Stockholm, Sweden
• OSTI ID: 6037161
• Journal Information: J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 48:2; ISSN JCEMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACROMEGALY
ANIMALS
ANTIBODIES
BLOOD SERUM
CHROMATOGRAPHY
DATA
DATA FORMS
DISEASES
ENDOCRINE DISEASES
EXPERIMENTAL DATA
GRAPHS
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MAN
NUMERICAL DATA
PATIENTS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES