Factors affecting growth and toxin production by Clostridium botulinum type E on irradiated (0. 3 Mrad) chicken skins

Journal Article · Sat May 01 00:00:00 EDT 1982 · J. Food Process. Preserv.; (United States)

DOI:https://doi.org/10.1111/j.1365-2621.1982.tb12733.x· OSTI ID:6842238

Firstenberg-Eden, R ^[1]; Rowley, D B; Shattuck, G E

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1. Bactomatic, Princeton, NJ

A model system (chicken skins with chicken exudate) was used to determine if Clostridium botulinum type E (Beluga) spores, stressed by low dose irradiation, would develop and produce toxin at abuse temperatures of 10 and 30/sup 0/C in the absence of characteristic spoilage. Unstressed spores germinated, multiplied, and produced toxin on vacuum-packed chicken skins, stored at either 30 or 10/sup 0/C. Cell numbers increased faster and toxin was evident sooner at 30/sup 0/C than at 10/sup 0/C. At 30/sup 0/C, growth occurred and toxin was produced more slowly when samples were incubated aerobically than anaerobically. When samples were incubated aerobically at 10/sup 0/C, no toxin was detected within a test period of 14 days. An irradiation dose of 0.3 Mrad at 5/sup 0/C reduced a spore population on vacuum-sealed chicken skins by about 90%. The surviving population produced toxin at 30/sup 0/C under either aerobic or anaerobic conditions, at 10/sup 0/C no toxin was detected even on skins incubated anaerobically. Under the worst conditions (30/sup 0/C, vacuum packed) toxin was not detected prior to characteristic spoilage caused by the natural flora surviving 0.3 Mrad.

• OSTI ID: 6842238
• Journal Information: J. Food Process. Preserv.; (United States), Journal Name: J. Food Process. Preserv.; (United States) Vol. 47:3; ISSN JFPPD
• Country of Publication: United States
• Language: English

Related Subjects

560132* -- Radiation Effects on Microorganisms-- Food Preservation-- (-1987)
560155 -- Radiation Effects on Animals-- Disinfestation-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROBIC CONDITIONS
ANAEROBIC CONDITIONS
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BIRDS
CLOSTRIDIUM
CLOSTRIDIUM BOTULINUM
FOWL
GROWTH
INHIBITION
IRRADIATION
LOW DOSE IRRADIATION
MATERIALS
MEDIUM TEMPERATURE
MICROORGANISMS
PRESERVATION
RADIATION EFFECTS
RADIOPRESERVATION
RADIOSENSITIVITY
TOXIC MATERIALS
TOXINS
VERTEBRATES