Cryogenic gamma irradiation of prototype pork and chicken and antagonistic effect between Clostridium botulinum types A and B

Journal Article · Thu Dec 01 04:00:00 EST 1977 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6801647

Anellis, A; Shattuck, E; Morin, M; Srisara, B; Qvale, S; Rowley, D B; Ross, Jr, E W

Inoculated, irradiated pork (2,300 cans) and chicken (2,000 cans) pack studies were performed to establish the 12D dose for these foods. Each can was inoculated with a mixture of 10(6) spores of each of 10 strains of Clostridium botulinum (five type A and five type B), or a total of 10(7) spores. The cans received a series of increasing doses of gamma rays (60Co) at -30 +/- 10 degrees C; they were incubated for 6 months at 30 +/- 2 degrees C and examined for swelling, toxicity, and recoverable botulinal cells. The highest rate of swelling for both foods occurred within the first week of incubation, and maximum swelling was observed within 4 to 5 weeks. The minimal experimental sterilizing dose (ESD) based on flat, nontoxic sterile cans was 3.0 less than ESD less than or equal to 3.2 Mrad for pork and 4.0 less than ESD less than or equal to 4.2 Mrad for chicken. An analysis of the partial spoilage data by extreme-value statistics indicated with 90% confidence that the rate of spore death in the two foods was not a normal distribution, but appeared to favor a shifted exponential function. Based on the latter distribution, and assuming one most resistant strain in the mixture of 10 used, the 12D dose computed to 4.37 Mrad, with a shoulder of 0.11, for pork and 4.27 Mrad, with a shoulder of 0.51 Mrad, for chicken.

• Research Organization: Army Natick Research and Development Command, MA
• OSTI ID: 6801647
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 34:6; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560132* -- Radiation Effects on Microorganisms-- Food Preservation-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLOSTRIDIUM
CLOSTRIDIUM BOTULINUM
COBALT 60
COBALT ISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
FOOD
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MEAT
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSTERILIZATION
SPORES
STERILIZATION
SURVIVAL TIME
TOXICITY
YEARS LIVING RADIOISOTOPES