Middle cerebral artery thrombosis: acute blood-brain barrier consequences

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · J. Neuropathol. Exp. Neurol.; (United States)

DOI:https://doi.org/10.1097/00005072-198807000-00005· OSTI ID:6839934

Dietrich, W D; Prado, R; Watson, B D; Nakayama, H

The effect of middle cerebral artery (MCA) thrombosis on the integrity of the blood-brain barrier (BBB) was studied in rats using horseradish peroxidase (HRP). Endothelial injury with subsequent platelet thrombosis was produced by means of a rose bengal-sensitized photochemical reaction, facilitated by irradiating the right proximal MCA segment with the focused beam of an argon laser. At 15 minutes following thrombosis formation, diffuse leakage of HRP was observed bilaterally within cortical and subcortical brain areas. Peroxidase extravasation was most dense within the territory of the occluded artery including neocortical areas and dorso-lateral striatum. Contralaterally, a similar distribution was observed but with less intense HRP leakage. Ultrastructural studies demonstrated an increase in permeability to HRP within arterioles, venules and capillaries. At these sites, the vascular endothelium contained HRP-filled pinocytotic vesicles and tubular profiles. Although less intense, bilateral HRP leakage was also observed following MCA stenosis or femoral artery occlusion. Endothelial-platelet interactions at the site of vascular injury may be responsible for releasing substances or neurohumoral factors which contribute to the acute opening of the BBB.

• Research Organization: Univ. of Miami School of Medicine, FL (USA)
• OSTI ID: 6839934
• Journal Information: J. Neuropathol. Exp. Neurol.; (United States), Journal Name: J. Neuropathol. Exp. Neurol.; (United States) Vol. 47:4; ISSN JNENA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ARTERIES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BLOOD-BRAIN BARRIER
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CEREBRAL ARTERIES
CHEMICAL REACTIONS
DISEASES
ELECTROMAGNETIC RADIATION
ENDOTHELIUM
ENZYMES
GAS LASERS
LASER RADIATION
LASERS
MAMMALS
MORPHOLOGICAL CHANGES
ORGANS
OXIDOREDUCTASES
PATHOGENESIS
PERMEABILITY
PEROXIDASES
PHOTOCHEMICAL REACTIONS
RADIATIONS
RATS
RODENTS
THROMBOSIS
TISSUES
ULTRASTRUCTURAL CHANGES
VASCULAR DISEASES
VERTEBRATES