Simple canine model of arterial thrombosis with endothelial injury suitable for investigation of thrombolytic agents
Journal Article
·
· J. Pharmacol. Methods; (United States)
Three separate studies were done to evaluate a new canine model of arterial thrombosis with endothelial injury. Endothelial injury was produced by exposing the luminal surface of a 2-cm segment of femoral artery to 100 degrees C saline for 5 min. There was no disruption of proximal or distal blood flow with this model, and thrombolysis was continuously monitored by measuring /sup 125/I-labelled fibrin gamma emissions from the thrombus. Study No. 1 showed that complete endothelial denudation was achieved with this model. Study No. 2 demonstrated 1) adherence of the experimentally induced thrombus to subendothelial connective tissue, and 2) endogenous thrombolysis of approximately 9% during the initial 2 h after thrombus formation. Study No. 3 tested the usefulness of the model for evaluating the thrombolytic efficacy of urokinase. Urokinase (30,000 U/Kg, bolus IV injection) caused 38 +/- 5.4% thrombolysis within 90 min of drug administration versus 5.9 +/- 2.4% for a saline-treated control group. We conclude that this model provides a technically simple and reproducible method for the laboratory investigation of thrombosis and thrombolysis in arteries with endothelial injury.
- Research Organization:
- Purdue Univ., W. Lafayette, IN (USA)
- OSTI ID:
- 6769707
- Journal Information:
- J. Pharmacol. Methods; (United States), Journal Name: J. Pharmacol. Methods; (United States) Vol. 19:4; ISSN JPMED
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MODELS
BLOOD FLOW
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HYDROLASES
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
THROMBOSIS
TISSUES
TRACER TECHNIQUES
UROKINASE
VASCULAR DISEASES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MODELS
BLOOD FLOW
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HYDROLASES
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
THROMBOSIS
TISSUES
TRACER TECHNIQUES
UROKINASE
VASCULAR DISEASES
VERTEBRATES