Product inhibition of Zymomonas mobilis: evidence for two inhibitors

Conference · Sat Jan 01 04:00:00 EST 1983 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:6838720

Worden, R M; Donaldson, T L; Shumate, S E

Batch-growth experiments were conducted in New Brunswick model C-30 fermenters having a 350-ml working volume. Temperature and pH values of 36/sup 0/C and 6.0, respectively, were maintained using automatic controllers and a 2M KOH titrant. The growth medium contained 10% (w/v) glucose, 0.5% yeast extract (Difco), 0.1% KH/sub 2/PO/sub 4/, 0.05% MgSO/sub 4/ x 7H/sub 2/O, 0.1% (NH/sub 4/)/sub 2/SO/sub 4/, and 0.01M succinate. These culture conditions were found in a previous study to provide maximal initial growth and ethanol production rates for Z. mobilis (ATCC 10988). Growth medium was prepared and autoclaved (121/sup 0/C for 25 min) with the sugar separate from the remaining solutes to prevent darkening of the solution. After autoclaving, all components were combined, and the medium was allowed to cool under a nitrogen atmosphere to maintain anaerobic conditions. The medium was then spiked with the desired amount of 95% ethanol just prior to inoculation with Z. mobilis. The reactors were then sampled periodically to monitor biomass and ethanol concentrations. 8 references, 2 figures, 1 table.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6838720
• Report Number(s): CONF-830567-
• Conference Information: Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 13
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BACTERIA
BATCH CULTURE
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
CULTURE MEDIA
ETHANOL
FERMENTATION
GROWTH
HYDROXY COMPOUNDS
INHIBITION
KINETICS
MATHEMATICAL MODELS
MICROORGANISMS
ORGANIC COMPOUNDS
PRODUCTION
QUANTITY RATIO
REACTION KINETICS
ZYMOMONAS MOBILIS