cap alpha. /sub i/-3 cDNA encodes the. cap alpha. subunit of G/sub k/, the stimulatory G protein of receptor-regulated K/sup +/ channels
cDNA cloning has identified the presence in the human genome of three genes encoding ..cap alpha.. subunits of pertussis toxin substrates, generically called G/sub i/. They are named ..cap alpha../sub i/-1, ..cap alpha../sub i/-2 and ..cap alpha../sub i/-3. However, none of these genes has been functionally identified with any of the ..cap alpha.. subunits of several possible G proteins, including pertussis toxin-sensitive G/sub p/'s, stimulatory to phospholipase C or A/sub 2/, G/sub i/, inhibitory to adenylyl cyclase, or G/sub k/, stimulatory to a type of K/sup +/ channels. The authors now report the nucleotide sequence and the complete predicted amino acid sequence of human liver ..cap alpha../sub i/-3 and the partial amino acid sequence of proteolytic fragments of the ..cap alpha.. subunit of human erythrocyte G/sub k/. The amino acid sequence of the proteolytic fragment is uniquely encoded by the cDNA of ..cap alpha../sub i/-3, thus identifying it as ..cap alpha../sub k/. The probable identity of ..cap alpha../sub i/-1 with ..cap alpha../sub p/ and possible roles for ..cap alpha../sub i/-2, as well as additional roles for ..cap alpha../sub i/-1 and ..cap alpha../sub i/-3 (..cap alpha../sub k/) are discussed.
- Research Organization:
- Baylor College of Medicine, Houston, TX (USA)
- OSTI ID:
- 6838327
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:14
- Country of Publication:
- United States
- Language:
- English
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RECOMBINANT DNA
DNA SEQUENCING
DNA-CLONING
TOXINS
AMINO ACID SEQUENCE
ATP
CATIONS
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GENES
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PHOSPHORUS 32
POTASSIUM COMPOUNDS
PROTEINS
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SULFUR 35
ALKALI METAL COMPOUNDS
ANIMALS
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BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
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DIGESTIVE SYSTEM
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EVEN-ODD NUCLEI
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IONS
ISOTOPES
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MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEI
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NUCLEOTIDES
ODD-ODD NUCLEI
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ORGANS
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550201* - Biochemistry- Tracer Techniques