Identification of a GTP-binding protein. cap alpha. subunit that lacks an apparent ADP-ribosylation site for pertussis toxin

Journal Article · Sun May 01 00:00:00 EDT 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.9.3066· OSTI ID:6208506

Fong, H K.W.; Yoshimoto, K K; Eversole-Cire, P; Simon, M I

Recent molecular cloning of cDNA for the ..cap alpha.. subunit of bovine transducin (a guanine nucleotide-binding regulatory protein, or G protein) has revealed the presence of two retinal-specific transducins, called T/sub r/ and T/sub c/, which are expressed in rod or cone photoreceptor cells. In a further study of G-protein diversity and signal transduction in the retina, the authors have identified a G-protein ..cap alpha.. subunit, which they refer to as G/sub z/..cap alpha.., by isolating a human retinal cDNA clone that cross-hybridizes at reduced stringency with bovine T/sub r/ ..cap alpha..-subunit cDNA. The deduced amino acid sequence of G/sub z/..cap alpha.. is 41-67% identical with those of other known G-protein ..cap alpha.. subunits. However, the 355-residue G/sub z/..cap alpha.. lacks a consensus site for ADP-ribosylation by pertussis toxin, and its amino acid sequence varies within a number of regions that are strongly conserved among all of the other G-protein ..cap alpha.. subunits. They suggest that G/sub z/..cap alpha.., which appears to be highly expressed in neural tissues, represents a member of a subfamily of G proteins that mediate signal transduction in pertussis toxin-insensitive systems.

• Research Organization: California Institute of Technology, Pasadena (USA)
• OSTI ID: 6208506
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MESSENGER-RNA
HYBRIDIZATION
PROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
RETINA
BIOCHEMISTRY
DNA-CLONING
GUANINE
MAN
MICE
NUCLEOTIDES
PHOSPHORUS 32
TOXINS
AMINES
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
CHEMISTRY
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
EYES
FACE
HEAD
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PURINES
RADIOISOTOPES
RNA
RODENTS
SENSE ORGANS
STRUCTURAL CHEMICAL ANALYSIS
TOXIC MATERIALS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques