Transcriptional regulation of ceruloplasmin gene expression during inflammation
Mixed sequence oligonucleotides were used to isolate a series of acute-phase human liver cDNA clones corresponding to the serum ..cap alpha../sub 2/-globulin ceruloplasmin. These clones were characterized, sequenced, and used to analyze changes in hepatic ceruloplasmin mRNA content during inflammation. In all species examined, hepatic ceruloplasmin mRNA content increased approximately 6-10-fold over control values within 24 h following the induction of inflammation. The mechanisms leading to this increase in hepatic ceruloplasmin mRNA content were studied following turpentine-induced inflammation in Syrian hamsters. Nuclear run-on assays demonstrated an increase in the relative rate of transcription of the ceruloplasmin gene within 3 h following induction, reaching maximum values by 18 h. Hepatic ceruloplasmin mRNA content increased 2-fold within 12 h following induction, reached maximum values by 24 h, and returned to control within 72 h. In contrast, serum ceruloplasmin concentration did not increase until 36 h, reached maximal levels by 120 h, and remained elevated for the course of the study. These data indicate that inflammation leads to a rapid increase in hepatic ceruloplasmin mRNA content. This increase is largely the result of increased ceruloplasmin gene transcription, but comparison of the relative rate of transcription and mRNA accumulation suggests that changes in ceruloplasmin mRNA turnover are also involved. In addition, translational and/or post-translational mechanisms must account for the observed changes in serum ceruloplasmin concentration seen during inflammation.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO (USA)
- OSTI ID:
- 6838148
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:13
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CERULOPLASMIN
GENE REGULATION
POST-TRANSLATION MODIFICATION
INFLAMMATION
BIOCHEMISTRY
RECOMBINANT DNA
DNA SEQUENCING
HAMSTERS
LIVER
MESSENGER-RNA
OLIGONUCLEOTIDES
PHOSPHORUS 32
TRANSCRIPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHEMISTRY
COMPLEXES
COPPER COMPLEXES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
GLANDS
GLOBULINS
GLOBULINS-ALPHA
ISOTOPES
LIGHT NUCLEI
MAMMALS
METALLOPROTEINS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
SYMPTOMS
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques