alpha. - and. beta. -adrenergic receptors in proximal tubules of rat kidney

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6834234

Sundaresan, P R; Fortin, T L; Kelvie, S L ^[1]

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1. Univ. of Rochester Medical Center, NY (USA)

Proximal tubules were isolated from the rat kidney by collagenase digestion of the cortical tissue followed by Percoll gradient centrifugation. Microscopic and hormone-stimulated adenylate cyclase activity studies proved the purity of the preparation. ({sup 3}H)Prazosin, ({sup 3}H)rauwolscine, and ({sup 125}I)iodocyanopindolol were used to identify and quantitate respectively the {alpha}{sub 1}-, {alpha}{sub 2}- and {beta}-adrenergic receptors. Proximal tubular (F{sub 4}) particulate fraction was compared against other cortical nephron segment (F{sub 1},F{sub 2}) fractions and the total collagenase-digested cortex particulate suspension (F{sub t}). Proximal tubules were enriched in {alpha}{sub 1}- and {alpha}{sub 2}-adrenergic receptors compared with. The fractions enriched in glomeruli and distal tubular segments had relatively low concentrations of {alpha}{sub 1}- and {alpha}{sub 2}-adrenergic receptors. Isoproterenol-stimulated adenylate cyclase activities in the different fractions corroborated well with the pattern suggested by the ({sup 125}I)iodocyanopindolol binding studies. The results suggest that whole-cortex preparation radioligand binding studies may reflect proximal tubular {alpha}{sub 1}- and {alpha}{sub 2}-adrenergic receptor changes quite well. They may, however, miss or give erroneous impressions about {beta}-adrenergic receptor changes occurring in different cortical nephron segments.

• OSTI ID: 6834234
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
CYCLASES
DRUGS
ENZYMES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KIDNEYS
KINETICS
LYASES
MAMMALS
MEMBRANE PROTEINS
MOLECULAR BIOLOGY
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PROTEINS
RADIORECEPTOR ASSAY
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUBULES
VERTEBRATES