alpha. - and. beta. -adrenergic receptors in proximal tubules of rat kidney

Journal Article · Sun Nov 01 00:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6834234

Sundaresan, P R; Fortin, T L; Kelvie, S L ^[1]

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1. Univ. of Rochester Medical Center, NY (USA)

Proximal tubules were isolated from the rat kidney by collagenase digestion of the cortical tissue followed by Percoll gradient centrifugation. Microscopic and hormone-stimulated adenylate cyclase activity studies proved the purity of the preparation. ({sup 3}H)Prazosin, ({sup 3}H)rauwolscine, and ({sup 125}I)iodocyanopindolol were used to identify and quantitate respectively the {alpha}{sub 1}-, {alpha}{sub 2}- and {beta}-adrenergic receptors. Proximal tubular (F{sub 4}) particulate fraction was compared against other cortical nephron segment (F{sub 1},F{sub 2}) fractions and the total collagenase-digested cortex particulate suspension (F{sub t}). Proximal tubules were enriched in {alpha}{sub 1}- and {alpha}{sub 2}-adrenergic receptors compared with. The fractions enriched in glomeruli and distal tubular segments had relatively low concentrations of {alpha}{sub 1}- and {alpha}{sub 2}-adrenergic receptors. Isoproterenol-stimulated adenylate cyclase activities in the different fractions corroborated well with the pattern suggested by the ({sup 125}I)iodocyanopindolol binding studies. The results suggest that whole-cortex preparation radioligand binding studies may reflect proximal tubular {alpha}{sub 1}- and {alpha}{sub 2}-adrenergic receptor changes quite well. They may, however, miss or give erroneous impressions about {beta}-adrenergic receptor changes occurring in different cortical nephron segments.

• OSTI ID: 6834234
• Journal Information: American Journal of Physiology; (USA), Vol. 253:5; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
MOLECULAR BIOLOGY
SYMPATHOMIMETICS
RADIORECEPTOR ASSAY
TUBULES
PHYSIOLOGY
AMP
BIOCHEMICAL REACTION KINETICS
CYCLASES
KIDNEYS
RATS
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
DRUGS
ENZYMES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LYASES
MAMMALS
MEMBRANE PROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
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RODENTS
TRACER TECHNIQUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques