Mutagenicity of nitro compounds in Salmonella typhimurium in the presence of flavin mononucleotide in a preincubation assay

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850130206· OSTI ID:6833050

Dellarco, V L ^[1]; Prival, M J ^[2]

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1. Environmental Protection Agency, Washington, DC (USA)
2. Food and Drug Administration, Washington, DC (USA)

A series of nitro compounds (18 aromatic and one aliphatic) was evaluated using a modification of the standard Salmonella typhimurium mutagenicity assay. A preincubation protocol was used with flavin mononucleotide (FMN) incorporated into the assay mixture to facilitate nitro reduction. Several aromatic nitro compounds (m-nitroaniline, p-nitroaniline, 2,6-dinitrotoluene, 2,4-dinitrotoluene, 2,3-dinitrotoluene, 1,8-dinitronaphthalene), which were negative or only weakly mutagenic when tested in the standard plate incorporation assay, showed FMN-dependent mutagenic responses with this procedure for some nitro compounds, the addition of FMN was not needed for the detection of mutagenicity in the modified protocol. Not all nitro compounds were positive using the preincubation procedure with FMN. The lack of mutagenicity, however, does not appear to be the result of the inability of the modified method to reduce nitro compounds, since it was found that reduction does occur under the assay conditions for the two nonmutagens evaluated for nitro reduction (nitrobenzene and p-nitrophenol). It is suggested that the modified protocol may be useful for evaluating the mutagenicity of many nitro compounds.

• OSTI ID: 6833050
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 13:2; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRIDINES
AMINES
AROMATICS
AZAARENES
AZINES
BACTERIA
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DOSE-RESPONSE RELATIONSHIPS
FLAVINES
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
MUTANTS
NITRO COMPOUNDS
NITROBENZENE
NITROPHENOL
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PYRIDINES
REDUCTION
REVERTANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING