Assays of three carcinogen/non-carcinogen chemical pairs for in vivo induction of chromosome aberrations, sister chromatid exchanges and micronuclei

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850140402· OSTI ID:6832975

McFee, A F; Lowe, K W ^[1]; Jauhar, P P; MacGregor, J T; Wehr, C M ^[2]

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1. Oak Ridge Associated Univ., TN (USA)
2. USDA Western Regional Research Center, Albany, CA (USA)

Three pairs of structurally similar carcinogenic/non-carcinogenic chemicals were tested for in vivo genotoxic activity in B6C3F1 mice. The carcinogenic/non-carcinogenic pairs, respectively, were o-toluidine hydrochloride/o-anthranilic acid, 4-chloro-o-phenylenediamine/4-nitro-o-phenylenediamine, and 3-(chloromethyl)/pyridine hydrochloride/2-(chloromethyl)pyridine hydrochloride. Bone marrow cells from mice given intraperitoneal injections of up to the maximum tolerated dose were evaluated for chromosomal aberration, sister chromatid exchange, and micronucleus induction, o-anthranilic acid and o-toluidine hydrochloride did not increase the frequency of chromosomal aberrations or micronuclei. o-Toluidine hydrochloride increased the frequency of sister chromatid exchanges in two successive trials, while o-anthranilic acid had a positive effect on sister chromatid exchanges in two of three trials. Both 2-(chloromethyl) and 3-(chloromethyl)pyridine hydrochloride were negative for all three endpoints. Assays for chromosomal aberrations and micronuclei each distinguished between 4-chloro-o-phenylenediamine and its non-carcinogenic companion, 4-nitro-o-phenylenediamine produced a few cells with very large numbers of aberrations rather than an even distribution of damage among cells.

• OSTI ID: 6832975
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 14:4; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTHRACENE
AROMATICS
AZAARENES
AZINES
BENZIDINE
BENZOPYRENE
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
CELL NUCLEI
CHLORINATED ALIPHATIC HYDROCARBONS
CHLOROFORM
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DRUGS
GENETIC EFFECTS
HALOGENATED ALIPHATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
IN VIVO
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MICE
MUTATION FREQUENCY
MUTATIONS
NITRO COMPOUNDS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIDINES
QUINOLINES
RODENTS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
URETHANE
VERTEBRATES