Microvascular effects of copper deficiency in rats

Journal Article · Wed Feb 15 00:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:6832641

Schuschke, D A; Saari, J T; Ackermann, D M; Miller, F N ^[1]

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1. Univ. of Louisville, KY (USA)

We have studied the microcirculatory responses in copper deficient rats using the rat cremaster muscle preparation. Male Sprague-Dawley rats were fed either a copper supplemented diet (CuS, 5 ppm) or a copper deficient diet (CuD, O ppm) for five weeks prior to experimentation. The rats (240-300g) were anesthetized with pentobarbital and the cremaster (with intact nerve and blood supply) were spread in a tissue bath filled with krebs solution. In vivo television microscopy was used to observe the microcirculation. Fluorescein isothiocyanate tagged to bovine serum albumin (FITC-BSA) was injected i.a. 30 min prior to the start of experimentation. In the CuS animals photoactivation of the intravascular FITC-BSA caused significant platelet aggregation and reduction in red blood cell column diameter (RBCCD) by 30 min and stasis of flow by 60 min. In CuD animals there was no reduction in RBCCD and only minor platelet aggregation after 60 min of photoactivation. Topical administration of compound 48/80 (1.0 and 10.0 {mu}g/ml) induced a significantly greater macromolecular leakage (increased interstitial fluorescence of FITC-BSA) in the CuD animals than in the control, CuS animals. These results suggest that copper deficiency results in marked alterations of the regulatory mechanisms governing thrombosis and inflammation.

• OSTI ID: 6832641
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Vol. 3:4; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COPPER
BIOLOGICAL EFFECTS
RATS
BLOOD CIRCULATION
DIET
INFLAMMATION
NUTRITIONAL DEFICIENCY
THROMBOSIS
ANIMALS
CARDIOVASCULAR DISEASES
DISEASES
ELEMENTS
MAMMALS
METALS
PATHOLOGICAL CHANGES
RODENTS
SYMPTOMS
TRANSITION ELEMENTS
VASCULAR DISEASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology