Proliferative kinetics and chromosome damage in trisomy 21 lymphocyte cultures exposed to gamma-rays and bleomycin

Journal Article · Sun Apr 01 04:00:00 EST 1984 · Cancer Res.; (United States)

OSTI ID:6832496

Morimoto, K; Kaneko, T; Iijima, K; Koizumi, A

Lymphocytes from patients with Down's syndrome (trisomy 21) have been investigated for cell cycle kinetics, cell proliferation delays, and chromosomal aberrations after exposure to gamma-rays or bleomycin. Analysis by sister chromatid differential staining revealed that trisomy 21 lymphocytes started cell cycling about 5 hr earlier than did normal diploid lymphocytes after phytohemagglutinin stimulation as a whole, but that cycling trisomic and normal cells had the same mean cell cycle times. When exposed to gamma-rays or bleomycin in G0, trisomy 21 lymphocytes showed a 30% or, on average, 50% longer duration of cell turnover times, respectively, than normal cells; only bleomycin-treated trisomic cells had a biphasic dose-response. Frequencies of dicentrics and rings in first-division cells after gamma-ray or bleomycin exposure were twice as high in trisomic cells as in normal cells. The frequency of aberrations decreased by 50% (gamma-ray-exposed) or 65 to 85% (bleomycin-treated) through successive divisions; trisomic cells showed a more marked decline in aberration yields compared to normal cells after bleomycin treatment. These data support the idea that circulating lymphocytes in trisomy 21 patients have a shorter average life span or a younger average age.

• Research Organization: Department of Public Health, Faculty of Medicine, University of Tokyo, Japan
• OSTI ID: 6832496
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:4; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLEOMYCIN
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CYCLE
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
CONGENITAL MALFORMATIONS
CONNECTIVE TISSUE CELLS
DISEASES
DOWNS SYNDROME
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HEREDITARY DISEASES
IONIZING RADIATIONS
LEUKOCYTES
LIFE SPAN
LYMPHOCYTES
MALFORMATIONS
MATERIALS
MUTATIONS
PATHOLOGICAL CHANGES
PATIENTS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS