Receptor-mediated release of endothelium-derived relaxing factor and prostacyclin from bovine aortic endothelial cells is coupled

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2334· OSTI ID:6828895

De Nucci, G; Gryglewski, R J; Warner, T D; Vane, J R ^[1]

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1. William Harvey Research Institute, London (England)

Bovine aortic endothelial cells were grown on microcarrier beads and were perfused with Krebs-Ringer solution. Endothelium-derived relaxing factor (EDRF) was bioassayed on a cascade of four strips of rabbit aorta, and prostacyclin was analyzed by RIA of 6-oxo-prostaglandin F{sub 1{alpha}}. The endothelial cells released EDRF and prostacyclin when stimulated with bradykinin and its analogues, or with ADP, ATP, arachidonic acid, and phospholipase C. The detection of EDRF was potentiated by superoxide dismutase, and the relaxation of rabbit aortic strips induced by EDRF was antagonized by methylene blue. The release of EDRF and prostacyclin was inhibited by phorbol myristate acetate, R59022 (a diacylglycerol kinase inhibitor), and gentamycin. The authors suggest that the release of EDRF and prostacyclin is coupled and the initial common step is activation of a phospholipase C.

• OSTI ID: 6828895
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:7, Issue 7; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NITRIC OXIDE
BIOLOGICAL EFFECTS
PHOSPHODIESTERASES
ENZYME INDUCTION
PROSTAGLANDINS
RADIOIMMUNOASSAY
RECEPTORS
CHEMICAL ACTIVATION
AORTA
CATTLE
ENDOTHELIUM
PHORBOL ESTERS
ANIMAL TISSUES
ANIMALS
ARTERIES
BIOASSAY
BLOOD VESSELS
BODY
CARCINOGENS
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
DIAGNOSTIC TECHNIQUES
DOMESTIC ANIMALS
ENZYMES
ESTERASES
ESTERS
GENE REGULATION
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE PROTEINS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RUMINANTS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques