Incision of damaged versus nondamaged DNA by the Escherichia coli UvrABC proteins

Journal Article · Thu Aug 25 04:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.16.7855· OSTI ID:6810793

Caron, P R; Grossman, L ^[1]

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1. Johns Hopkins Univ. School of Hygiene and Public Health, Baltimore, MD (USA)

Incision of damaged DNA by the Escherichia coli UvrABC endonuclease requires the UvrA, UvrB, and UvrC proteins as well as ATP hydrolysis. This incision reaction can be divided into three steps: site recognition, preincision complex formation, and incision. UvrAB is able to execute the first two steps in the reaction while the addition of UvrC is required for the incision of DNA. This incision reaction does not require ATP hydrolysis and results in the formation of a tight UvrABC post-incision complex and the generation of an oligomer of approximately 12 nucleotides. At high UvrABC concentrations the specificity of the incision for damaged DNA is decreased and significant incision of undamaged DNA occurs. Analogous to damage specific incision, this type of incision leads to generation of an oligonucleotide, but in this case the size is approximately 9 nucleotides in length. Further evidence shows that the combination of UvrB and UvrC proteins can generate a significant amount of a similar size product on undamaged DNA. In addition, the UvrC protein alone can generate a small amount of the same product. Immunological characterization of the weak nuclease activity seen with UvrC indicates that the activity is very tightly associated with the purified UvrC protein.

• OSTI ID: 6810793
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:16; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ATP
BACTERIA
BIOCHEMISTRY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DNA
DNA POLYMERASES
DNA REPAIR
DNA-ASE
ELECTROPHORESIS
ENDONUCLEASES
ENZYMATIC HYDROLYSIS
ENZYMES
ESCHERICHIA COLI
ESTERASES
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
LYSIS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PYRIMIDINE DIMERS
RECOVERY
REPAIR
SOLVOLYSIS
TRANSFERASES
TRITIUM COMPOUNDS