DNA repair by UvrABC: In vitro analysis of the preincision complex
Conference
·
OSTI ID:134868
- Gorlaeus Labs., Leiden (Netherlands); and others
The UvrABC endonuclease repairs a variety of structurally different lesions. The preincision complex consisting of UvrB and damaged DNA plays a central role in successful damage recognition by UvrA and UvrB, and is a prerequisite for the subsequent processing in the presence of UvrC, resulting in dual incisions of the DNA. Although there is still an effect on efficiency, the type of lesion seems to have only a minor influence on the mechanism of repair, since incisions occur at fixed distances from each lesion. Therefore, an important aspect of unraveling the molecular mechanisms of UvrABC-dependent DNA repair is to understand how this system is able to process different lesions in a similar way. Attention in this work has been focused on which domains of UvrB are specifically involved in the formation of the UvrB-DNA preincision complex and on the structure of this complex.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134868
- Report Number(s):
- CONF-9307221--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Incision of damaged versus nondamaged DNA by the Escherichia coli UvrABC proteins
Repair of DNA-containing pyrimidine dimers
Recognition and repair of 2-aminofluorene- and 2-(acetylamino)fluorene-DNA adducts by UVRABC nuclease
Journal Article
·
Thu Aug 25 00:00:00 EDT 1988
· Nucleic Acids Research; (UK)
·
OSTI ID:6810793
Repair of DNA-containing pyrimidine dimers
Journal Article
·
Mon Aug 01 00:00:00 EDT 1988
· FASEB J.; (United States)
·
OSTI ID:6769036
Recognition and repair of 2-aminofluorene- and 2-(acetylamino)fluorene-DNA adducts by UVRABC nuclease
Journal Article
·
Tue Jul 11 00:00:00 EDT 1989
· Biochemistry; (USA)
·
OSTI ID:5060369