Spatial heterogeneity of metabolism in skeletal muscle in vivo studied by sup 31 P-NMR spectroscopy

Journal Article · Mon Feb 29 23:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6809843

Challiss, R A.J.; Blackledge, M J; Radda, G K ^[1]

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1. Univ. of Oxford (England)

Phase modulated rotating-frame imaging, a localization technique for phosphorus nuclear magnetic resonance spectroscopy, has been applied to obtain information on heterogeneity of phosphorus-containing metabolites in skeletal muscle of the rat in vivo. The distal muscles of the rat hindlimb have been studied at rest and during steady-state isometric twitch contraction; the use of a transmitter surface coil and an electrically isolated, orthogonal receiver Helmholtz coil ensure accurate spatial assignment (1 mm resolution). At rest, intracellular pH was higher and PCr/(PCr + P{sub i}) was lower in deeper muscle compared with superficial muscle of the distal hindlimb. Upon steady-state stimulation, the relatively more alkaline pH of deep muscle was maintained, whereas greater changes in PCr/(PCr + P{sub i}) and P{sub i}/ATP occurred in the superficial muscle layer. This method allows rapid (75 min for each spectral image) acquisition of quantitative information on metabolic heterogeneity in vivo.

• OSTI ID: 6809843
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:3; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ATP
BODY
BODY AREAS
CARBOXYLIC ACIDS
IN VIVO
ISOTOPES
LIGHT NUCLEI
LIMBS
MAGNETIC RESONANCE
MAMMALS
METABOLISM
METABOLITES
MUSCLES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PH VALUE
PHOSPHOCREATINE
PHOSPHORUS 31
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RATS
RESOLUTION
RESONANCE
RODENTS
SPATIAL RESOLUTION
STABLE ISOTOPES
VERTEBRATES