In vivo sup 31 P-NMR spectroscopy of chronically stimulated canine skeletal muscle

Journal Article · Mon Feb 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6870113

Clark, III, B J; McCully, A K; Subramanian, H V; Hammond, R L; Salmons, S; Chance, B; Stephenson, L W ^[1]

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1. Children's Hospital of Philadelphia, Harrison (USA) Univ. of Pennsylvania School of Medicine, Philadelphia (USA) Univ. of Birmingham (England)

Chronic stimulation converts skeletal muscle of mixed fiber type to a uniform muscle made up of type I, fatigue-resistant fibers. Here, the bioenergetic correlates of fatigue resistance in conditioned canine latissimus dorsi are assessed with in vivo phosphorus-31 nuclear magnetic resonance ({sup 31}P-NMR) spectroscopy. After chronic electrical stimulation, five dogs underwent {sup 31}P-NMR spectroscopic and isometric tension measurements on conditioned and contralateral control muscle during stimulation for 200, 300, 500, and 800 ms of an 1,100-ms duty cycle. With stimulation, phosphocreatine (PCr) fell proportional to the degree of stimulation in both conditioned and control muscle but fell significantly less in conditioned muscle at all the least intense stimulation period (200 ms). Isometric tension, expressed as a tension time index per gram muscle, was significantly greater in the conditioned muscle at the two longest stimulation periods. The overall small change in PCr and the lack of a plateau in tension observed in the conditioned muscle are similar to that seen in cardiac muscle during increased energy demand. This study indicates that the conditioned muscle's markedly enhanced resistance to fatigue is in part the result of its increased capacity for oxidative phosphorylation.

• OSTI ID: 6870113
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:2; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID ANHYDRASES
ANIMALS
ATP-ASE
BEAGLES
BIOELECTRICITY
BIOLOGICAL FATIGUE
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
DOGS
ELECTRICITY
ELECTROPHORESIS
ENZYMES
ERYTHROCYTES
EXERCISE
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MATERIALS
MUSCLES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
OXIDATION
PHOSPHOHYDROLASES
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PHYSIOLOGY
RECOVERY
RESONANCE
STABLE ISOTOPES
STIMULATION
VERTEBRATES