Tenax-GC extraction technique for residual polychlorinated biphenyl and polyaromatic hydrocarbon analysis in biodegradation assays
A rapid Tenax-GC extraction technique has been evaluated for use in conjunction with aqueous biodegradation assays for polyaromatic hydrocarbons and polychlorinated biphenyls. The method was quantitatively efficient and reproducible for phenanthrene, but variable and not quantitative for Aroclor 1254 (polychlorinated biphenyls). Aqueous sample volumes and varying concentrations of organic matter influenced polychlorinated biphenyl and polyaromatic hydrocarbon extraction efficiency. Phenanthrene recovery was decreased by soil extract but unaffected by spent bacteriological culture medium. Both types of organic matter cused significant reduction of Aroclor 1254 recovery. Polyaromatic hydrocarbon and polychlorinated biphenyl biodegradation assays, performed with reservoir samples, supported the laboratory evaluation. The study demonstrated the utility of the Tenax-GC extraction technique for phenanthrene analysis in biodegradation assessment; however, Tenax-GC extraction was not appropriate for Aroclor 1254 biodegradation studies.
- Research Organization:
- Univ. of Tennessee, Knoxville
- OSTI ID:
- 6809333
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 39:1; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BIODEGRADATION
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
EXTRACTION
HYDROCARBONS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHENANTHRENE
POLYCYCLIC AROMATIC HYDROCARBONS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES