Binding of mammalian ribosomes to MS2 phage RNA reveals an overlapping gene encoding a lysis function
Journal Article
·
· Cell; (United States)
- Cold Spring Harbor Lab, NY
The main binding site for mammalian ribosomes on the single-stranded RNA of bacteriophage MS2 is located nine tenths of the way through the coat protein gene. Translation initiated at an AUG triplet in the +1 frame yields a 75 amino acid polypeptide which terminates within the synthetase gene at a UAA codon, also in the +1 frame. Analysis of the translation products directed by RNA from op3, a UGA nonsense mutant of phage f2, identifies the overlapping cistron as a lysis gene. In this paper we show that the op3 mutation is a C ..-->.. U transition occurring in the second codon of the synthetase cistron. We discuss the properties of the overlapping gene in relation to its lysis function, recognition of the lysis initiator region by E. coli versus eucaryotic ribosomes and op3 as a ribosome binding site mutant for the f2 synthetase cistron.
- OSTI ID:
- 6809312
- Journal Information:
- Cell; (United States), Journal Name: Cell; (United States) Vol. 18; ISSN CELLB
- Country of Publication:
- United States
- Language:
- English
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