Sequence-specific cleavage of double helical DNA by triple helix formation

Journal Article · Thu Oct 29 23:00:00 EST 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.3118463· OSTI ID:6806917

Moser, H E; Dervan, P B

Homopyrimidine oligodeoxyribonucleotides with EDTA x Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA x Fe at the 5' end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.

• Research Organization: California Institute of Technology, Pasadena (USA)
• OSTI ID: 6806917
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 238; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
AMINO ACIDS
AZINES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHELATING AGENTS
CLEAVAGE
CONFIGURATION
CRYSTAL STRUCTURE
DNA
DNA MISMATCH
DNA SEQUENCING
EDTA
ELEMENTS
GENETIC MAPPING
HELICAL CONFIGURATION
HETEROCYCLIC COMPOUNDS
IRON
KINETICS
MAPPING
METALS
MICROSTRUCTURE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
REACTION KINETICS
STRAND BREAKS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENTS