Site-specific cleavage of a yeast chromosome by oligonucleotide-directed triple-helix formation
- California Institute of Technology, Pasadena (USA)
Oligonucleotides equipped with EDTA{center dot}Fe can bind specifically to duplex DNA by triple-helix formation and produce double-strand cleavage at binding sites greater than 12 base pairs in size. To demonstrate that oligonucleotide-directed triple-helix formation is a viable chemical approach for the site-specific cleavage of large genomic DNA, an oligonucleotide with EDTA{center dot}Fe at the 5{prime} and 3{prime} ends was targeted to a 20-base pair sequence din the 340-kilobase pair chromosome III of Saccharomyces cerevisiae. Double-strand cleavage products of the correct size and location were observed, indicating that the oligonucleotide bound and cleaved the target site among almost 14 megabase pairs of DNA. Because oligonucleotide-directed triple-helix formation has the potential to be a general solution for DNA recognition, this result has implications for physical mapping of chromosomes.
- OSTI ID:
- 6158867
- Journal Information:
- Science (Washington, D.C.); (USA), Vol. 249:4964; ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
Similar Records
Sequence-specific cleavage of double helical DNA by triple helix formation
Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation
Related Subjects
CHROMOSOMES
GENETIC MAPPING
OLIGONUCLEOTIDES
BIOCHEMICAL REACTION KINETICS
CLEAVAGE
DNA
EDTA
GENES
HELICAL CONFIGURATION
RECEPTORS
SACCHAROMYCES CEREVISIAE
AMINO ACIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CONFIGURATION
CRYSTAL STRUCTURE
EUMYCOTA
FUNGI
KINETICS
MAPPING
MEMBRANE PROTEINS
MICROORGANISMS
MICROSTRUCTURE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
REACTION KINETICS
SACCHAROMYCES
YEASTS
550400* - Genetics