Site-specific cleavage of a yeast chromosome by oligonucleotide-directed triple-helix formation

Journal Article · Fri Jul 06 04:00:00 EDT 1990 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.2195655· OSTI ID:6158867

Strobel, S A; Dervan, P B ^[1]

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1. California Institute of Technology, Pasadena (USA)

Oligonucleotides equipped with EDTA{center dot}Fe can bind specifically to duplex DNA by triple-helix formation and produce double-strand cleavage at binding sites greater than 12 base pairs in size. To demonstrate that oligonucleotide-directed triple-helix formation is a viable chemical approach for the site-specific cleavage of large genomic DNA, an oligonucleotide with EDTA{center dot}Fe at the 5{prime} and 3{prime} ends was targeted to a 20-base pair sequence din the 340-kilobase pair chromosome III of Saccharomyces cerevisiae. Double-strand cleavage products of the correct size and location were observed, indicating that the oligonucleotide bound and cleaved the target site among almost 14 megabase pairs of DNA. Because oligonucleotide-directed triple-helix formation has the potential to be a general solution for DNA recognition, this result has implications for physical mapping of chromosomes.

• OSTI ID: 6158867
• Journal Information: Science (Washington, D.C.); (USA), Journal Name: Science (Washington, D.C.); (USA) Vol. 249:4964; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMOSOMES
CLEAVAGE
CONFIGURATION
CRYSTAL STRUCTURE
DNA
EDTA
EUMYCOTA
FUNGI
GENES
GENETIC MAPPING
HELICAL CONFIGURATION
KINETICS
MAPPING
MEMBRANE PROTEINS
MICROORGANISMS
MICROSTRUCTURE
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
REACTION KINETICS
RECEPTORS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
YEASTS