Cloning, sequencing, and expression of the gene coding for the human platelet. cap alpha. /sub 2/-adrenergic receptor

Journal Article · Fri Oct 30 00:00:00 EST 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.2823383· OSTI ID:6805416

Kobilka, B K; Matsui, H; Kobilka, T S; Yang-Feng, T L; Francke, U; Caron, M G; Lefkowitz, R J; Regan, J W

The gene for the human platelet ..cap alpha../sub 2/-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of ..cap alpha../sub 2/-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human ..beta../sub 2/- and ..beta../sub 1/-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional ..cap alpha../sub 2/-adrenergic receptor subtypes.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA)
• OSTI ID: 6805416
• Journal Information: Science (Washington, D.C.); (United States), Vol. 238
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
CLONING
DNA SEQUENCING
RECEPTORS
AMINO ACID SEQUENCE
SYMPATHOMIMETICS
GENE REGULATION
BLOOD PLATELETS
FROGS
LIGANDS
MAN
OOCYTES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DRUGS
GERM CELLS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry