Cloning and expression of a human kidney cDNA for an /alpha//sub 2/-adrenergic receptor subtype
Journal Article
·
· Proc. Natl. Acad. Sci. USA; (United States)
An /alpha//sub 2/-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet /alpha//sub 2/-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet /alpha//sub 2/-adrenergic receptor and is consistent with the structure of other members of he family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the /alpha//sub 2/-adrenergic ligand (/sup 3/H)rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the /alpha//sub 2/B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet /alpha//sub 2/-adrenergic receptor (/alpha//sub 2/A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective /alpha/-adrenergic ligands.
- Research Organization:
- Howard Hughes Medical Institute, Durham, NC (USA)
- OSTI ID:
- 5822662
- Journal Information:
- Proc. Natl. Acad. Sci. USA; (United States), Journal Name: Proc. Natl. Acad. Sci. USA; (United States) Vol. 85:17; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning and expression of a rat brain. alpha. sub 2B -adrenergic receptor
Expansion of the. alpha. sub 2 -adrenergic receptor family: Cloning and characterization of a human. alpha. sub 2 -adrenergic receptor subtype, the gene for which is located on chromosome 2
Cloning, sequencing, and expression of the gene coding for the human platelet. cap alpha. /sub 2/-adrenergic receptor
Journal Article
·
Thu Jan 31 23:00:00 EST 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5043261
Expansion of the. alpha. sub 2 -adrenergic receptor family: Cloning and characterization of a human. alpha. sub 2 -adrenergic receptor subtype, the gene for which is located on chromosome 2
Journal Article
·
Sun Jul 01 00:00:00 EDT 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:6214411
Cloning, sequencing, and expression of the gene coding for the human platelet. cap alpha. /sub 2/-adrenergic receptor
Journal Article
·
Thu Oct 29 19:00:00 EST 1987
· Science (Washington, D.C.); (United States)
·
OSTI ID:6805416
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
KIDNEYS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MAN
MAPPING
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RECEPTORS
RECOMBINANT DNA
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
KIDNEYS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MAN
MAPPING
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RECEPTORS
RECOMBINANT DNA
TRITIUM COMPOUNDS
VERTEBRATES