Electrolyte composition of renal tubular cells in gentamicin nephrotoxicity

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Kidney Int.; (United States)

DOI:https://doi.org/10.1038/ki.1988.118· OSTI ID:6805038

Matsuda, O; Beck, F X; Doerge, A T; Thurau, K

The effect of long-term gentamicin administration on sodium, potassium, chloride and phosphorus concentrations was studied in individual rat renal tubular cells using electron microprobe analysis. Histological damage was apparent only in proximal tubular cells. The extent of damage was only mild after 7 days of gentamicin administration (60 mg/kg body wt/day) but much more pronounced after 10 days. GFR showed a progressive decline during gentamicin treatment. In non-necrotic proximal tubular cells, sodium was increased from 14.6 +/- 0.3 (mean +/- SEM) in controls to 20.6 +/- 0.4 after 7 and 22.0 +/- 0.8 mmol/kg wet wt after 10 days of gentamicin administration. Chloride concentration was higher only after 10 days (20.6 +/- 0.6 vs. 17.3 +/- 0.2 mmol/kg wet wt). Both cell potassium and phosphorus concentrations were diminished by 6 and 15, and by 8 and 25 mmol/kg wet wt after 7 and 10 days of treatment, respectively. In contrast, no major alterations in distal tubular cell electrolyte concentrations could be observed after either 7 or 10 days of gentamicin administration. As in proximal tubular cells, distal tubular cell phosphorus concentrations were, however, lowered by gentamicin treatment. These results clearly indicate that gentamicin exerts its main effect on proximal tubular cells. Decreased potassium and increased sodium and chloride concentrations were observed in proximal tubular cells exhibiting only mild histological damage prior to the onset of advanced tissue injury. Necrotic cells, on the other hand, showed widely variable intracellular electrolyte concentration patterns.

• Research Organization: Tokyo Medical and Dental Univ. (Japan)
• OSTI ID: 6805038
• Journal Information: Kidney Int.; (United States), Journal Name: Kidney Int.; (United States) Vol. 33:6; ISSN KDYIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BODY
BODY COMPOSITION
CHEMICAL ANALYSIS
DISEASES
DRUGS
ELECTROLYTES
ELECTRON MICROPROBE ANALYSIS
KIDNEYS
MAMMALS
MICROANALYSIS
NECROSIS
ORGANS
PATHOLOGICAL CHANGES
RATS
RODENTS
TOXICITY
UROGENITAL SYSTEM DISEASES
VERTEBRATES