Norepinephrine metabolism in neuronal cultures is increased by angiotensin II
In this study the authors have examined the actions of angiotensin II (ANG II) on catecholamine metabolism in neuronal brain cell cultures prepared from the hypothalamus and brain stem. Neuronal cultures prepared from the brains of 1-day-old Sprague-Dawley rats exhibit specific neuronal uptake mechanisms for both norepinephrine (NE) and dopamine (DA), and also monoamine oxidase (MAO) and catechol O-methyltransferase (COMT) activity. Separate neuronal uptake sites for NE and DA were identified by using specific neuronal uptake inhibitors for each amine. In previous studies, they determined that ANG II (10 nM-1 ..mu..M) stimulates increased neuronal (/sup 3/H)NE uptake by acting as specific receptors. They have confirmed these results here and in addition have shown that ANG II has not significant effects on neuronal (/sup 3/H)DA uptake. These results suggest that the actions of ANG II are restricted to the NE transporter in neuronal cultures. It is possible that ANG II stimulates the intraneuronal metabolism of at least part of the NE that is taken up, because the peptide stimulates MAO activity, an effect mediated by specific ANG II receptors. ANG II had no effect on COMT activity in neuronal cultures. Therefore, the use of neuronal cultures of hypothalamus and brain stem they have determined that ANG II can specifically alter NE metabolism in these areas, while apparently not altering DA metabolism.
- Research Organization:
- Univ. of Florida, Gainesville (USA)
- OSTI ID:
- 6804879
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:6; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Neurotransmitter mechanisms of the action of the antihistamine dimebon on the brain
Norepinephrine uptake by rat jejunum: Modulation by angiotensin II
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINES
ANGIOTENSIN
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DOPAMINE
DRUGS
ENZYME ACTIVITY
ENZYMES
GLOBULINS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHENOLS
POLYPHENOLS
PROTEINS
RADIOENZYMATIC ASSAY
RATS
RODENTS
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
UPTAKE
VASOCONSTRICTORS
VERTEBRATES