Submicrogram determination of lanthanides through quenching of calcein blue fluorescence
It has been found that trace levels of lanthanide ions efficiently quench the fluorescence of calcein blue and that an analytical method based on this quenching is far more sensitive (0.01-0.02 ..mu..g/mL depending on the identity of the lanthanide ion) than existing methods based on absorption spectrophotometry. The sensitivity levels are comparable to those noted for fluorescence observation of the few lanthanides which luminesce directly, but the calcein blue method may be applied equally well to any lanthanide ion. Interference by divalent transition-metal ions has been noted, but the lower degrees of quenching efficiency by these ions ensure that significant interference will exist only at relatively high metal levels.
- Research Organization:
- Squibb Institute for Medical Research, New Brunswick, NJ
- OSTI ID:
- 6804048
- Journal Information:
- Anal. Chem.; (United States), Vol. 59:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CERIUM
FLUORESCENCE SPECTROSCOPY
COBALT
COPPER
DYSPROSIUM
ERBIUM
EUROPIUM
GADOLINIUM
HOLMIUM
IRON
LANTHANUM
LUTETIUM
MANGANESE
NEODYMIUM
NICKEL
PRASEODYMIUM
SAMARIUM
TERBIUM
THULIUM
YTTERBIUM
ZINC
EXPERIMENTAL DATA
INTERFERING ELEMENTS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONLESS DECAY
TRACE AMOUNTS
CHEMICAL ANALYSIS
DATA
DE-EXCITATION
ELEMENTS
EMISSION SPECTROSCOPY
ENERGY TRANSFER
ENERGY-LEVEL TRANSITIONS
INFORMATION
METALS
NUMERICAL DATA
RARE EARTHS
SPECTROSCOPY
TRANSITION ELEMENTS
400102* - Chemical & Spectral Procedures