Muscarinic receptor subtypes mediating the mucosal response to neural stimulation of guinea pig ileum

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6803254

Carey, H V; Tien, X Y; Wallace, L J; Cooke, H J

Muscarinic receptors involved in the secretory response evoked by electrical stimulation of submucosal neutrons were investigated in muscle-stripped flat sheets of guinea pig ileum set up in flux chambers. Neural stimulation produced a biphasic increase in short-circuit current due to active chloride secretion. Atropine and 4-diphenylacetoxy-N-methylpiperadine methiodide (4-DAMP) (10/sup -7/ M) were more potent inhibitors of the cholinergic phase of the response than was pirenzepine. Dose-dependent increases in base-line short-circuit current were evoked by carbachol and bethanechol; 4-hydroxy-2-butynyl trimethylammonium chloride (McN A343) produced a much smaller effect. Tetrodotoxin abolished the effects of McN A343 but did not alter the responses of carbachol and bethanechol. McN A343 significantly reduced the cholinergic phase of the neurally evoked response and caused a rightward shift of the carbachol dose-response curve. All muscarinic compounds inhibited (/sup 3/H)quinuclidinyl benzilate binding to membranes from muscosal scrapings, with a rank order of potency of 4-DAMP > pirenzepine > McN A343 > carbachol > bethanechol. These results suggest that acetylcholine released from submucosal neurons mediates chloride secretion by interacting with muscarinic cholinergic receptors that display a high binding affinity for 4-DAMP. Activation of neural muscarinic receptors makes a relatively small contribution to the overall secretory response.

• Research Organization: Ohio State Univ., Columbus (USA)
• OSTI ID: 6803254
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560400 -- Other Environmental Pollutant Effects
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALOIDS
ANIMAL CELLS
ANIMALS
ANTIGENS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CHARGED PARTICLES
CHEMICAL ACTIVATION
CHLORIDES
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRIC FIELDS
GASTROINTESTINAL TRACT
GUINEA PIGS
HALIDES
HALOGEN COMPOUNDS
HYDROXY COMPOUNDS
INTESTINES
IONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
MUCOUS MEMBRANES
NERVE CELLS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PROTEINS
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
RODENTS
SECRETION
SMALL INTESTINE
SOMATIC CELLS
SYMPATHOMIMETICS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES