Configurational statistics of acyl chains in polyunsaturated lipid bilayers from /sup 2/H NMR

Journal Article · Wed Apr 29 00:00:00 EDT 1987 · J. Am. Chem. Soc.; (United States)

DOI:https://doi.org/10.1021/ja00243a010· OSTI ID:6802864

Salmon, A; Dodd, S W; Williams, G D; Beach, J M; Brown, M F

The gain a better understanding of the biological roles of polyunsaturated phospholipids, deuterium (/sup 2/H) NMR studies have been conducted of 1-perdeuteriopalmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, an asymmetric or mixed-chain saturated-polyunsaturated phospholipid, in the liquid crystalline (L/sub ..cap alpha../) phase. The palmitoyl (16:0) chain at the glycerol sn-1 position was labeled with /sup 2/H by perdeuteration, whereas the polyunsaturated, docosahexaenoyl (22:6 omega 3) chain at the sn-2 position was unlabeled, i.e., protiated. The /sup 2/H NMR results were compared to studies of 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphocholine, in which both the sn-1 and sn-2 palmitoyl chains were perdeuterated, as well as 1-palmitoyl-2-perdeuteriopalmitoyl-sn-glycero-3-phosphocholine, in which only the sn-2 chain was perdeuterated. Multilamellar phospholipid dispersions containing 50 wt % H/sub 2/O were employed, and /sup 2/H NMR spectra were obtained using quadrupolar echo methods at a magnetic field strength of 8.5 T. The experimental /sup 2/H NMR spectra were numerically deconvoluted (de-Paked) to yield subspectra corresponding to the parallel bilayer orientation with respect to the main applied magnetic field. The increased resolution of the de-Paked subspectra enabled profiles of the segmental order parameters of the individual C-/sup 2/H bonds, denoted by absolute value of S/sub CD/(i), to be derived as a function of chain position. Significant differences in the /sup 2/H NMR spectra and derived absolute value of S/sub CD/(i) profiles of the per-/sup 2/H-16:0 chains of the polyunsaturated and saturated bilayers were found.

• Research Organization: Univ. of Virginia, Charlottesville
• OSTI ID: 6802864
• Journal Information: J. Am. Chem. Soc.; (United States), Journal Name: J. Am. Chem. Soc.; (United States) Vol. 109:9; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
CHEMICAL BONDS
DATA
DEUTERIUM
ESTERS
EXPERIMENTAL DATA
HYDROGEN ISOTOPES
INFORMATION
ISOTOPES
LAYERS
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RESONANCE
SPECTRA
STABLE ISOTOPES