Bilayers of arachidonic acid containing phospholipids studied by sup 2 H and sup 31 P NMR spectroscopy

Journal Article · Tue Apr 30 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00231a015· OSTI ID:5596702

Rajamoorthi, K; Brown, M F ^[1]

---

1. Univ. of Arizona, Tucson (USA)

The configurational properties and dynamics of the arachidonic acyl chains of phospholipid bilayers have been investigated for the first time by solid-state {sup 2}H NMR techniques, with the goal of achieving a better understanding of the biological roles of polyunsaturated phospholipids. Vinyl perdeuterated arachidonic acid (20:4{sup {Delta}5,8,11,14}-d{sub 8}) was prepared from eicosatetraynoic acid (ETYA) and was esterified with 1-palmitoyl-sn-glycero-3-phosphocholine to yield 1-palmitoyl-2-vinylperdeuterioarachidonoyl-sn-glycero-3-phosphocholine ((16:0)(20:4-d{sub 8})PC). {sup 31}P NMR spectra of aqueous dispersions of (16:0)(20:4-d{sub 8})PC as well as 1-perdeuteriopalmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine ((per-{sup 2}H-16:0)(20:4)PC) were characteristic of the lamellar liquid-crystalline state. The dispersions had similar {sup 31}P chemical shift anisotropies, with little apparent motional averaging of the lineshapes due to macroscopic reorientation of liposomes or lateral diffusion of phospholipids about their curved surfaces. Comparison to other phosphatidylcholines indicated that both samples comprised the fully hydrated L{sub alpha} phase plus excess water. Simple models for interpretation of the findings are briefly mentioned and discussed. The results indicate clearly that the structural and dynamic properties of polyunsaturated acyl chains differ from those of saturated and monounsaturated chains in phospholipid bilayers.

• OSTI ID: 5596702
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:17; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANISOTROPY
ARACHIDONIC ACID
CARBOXYLIC ACIDS
CHEMICAL SHIFT
DEUTERIUM COMPOUNDS
ESTERS
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MATHEMATICAL MODELS
MEMBRANES
MOLECULAR MODELS
MONOCARBOXYLIC ACIDS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES